protoscript m mulv taq rt pcr kit  (New England Biolabs)

Journal: EMBO Reports

Article Title: Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation

doi: 10.1038/s44319-024-00152-3

Figure Lengend Snippet: Reagents and tools table

Article Snippet: RNA was isolated using TRIzol (Life Technologies) or TRI-reagent (Molecular Research Center, Inc), following the manufacturer’s instruction, and quantified by NanoDrop (Thermo Fisher). cDNA was synthesized from 1 μg of RNA using the ProtoScript TM M-MuLV Taq RT-PCR kit and random primers (New England BioLabs).

Techniques: Isolation, Recombinant, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Sequencing, shRNA, Control, Adjuvant, cDNA Synthesis, Software, Western Blot, Real-time Polymerase Chain Reaction, Imaging

Journal: Pharmaceutics

Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma

doi: 10.3390/pharmaceutics16040566

Figure Lengend Snippet: Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time RT-PCR analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).

Article Snippet: After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 µg of total RNA was used for first-strand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA).

Techniques: Expressing, Flow Cytometry, Confocal Microscopy, Western Blot, Quantitative RT-PCR, Binding Assay, Comparison, Labeling, Fluorescence, Software